Application of Electrical Mapping System in the Ex Vivo Whole Heart

Electrical Mapping System

Using multi-site recording electrodes placed in direct contact with excitable tissues or cells, electrical signals are acquired simultaneously from multiple locations. Dedicated software then processes these signals to generate visual activation maps and dynamic videos, enabling accurate identification, evaluation, and spatial localization of cardiac excitation activity. 

Simultaneous recording of atrial and ventricular electrical activity

Activation map of field potential (FP) across multiple sites. Conduction propagates from the red region (0 ms, earliest activation) to the blue region (5.8 ms, latest activation), with a total conduction time of 5.8 ms.

Activation map of field potential at
different sites

Sequential waveforms of excitation times at different sites 

Measurable parameters: excitation rate; pacemaker site localization; conduction direction and velocity; conduction dispersion; repolarization dispersion; QT interval dispersion. In addition to field potential recording, the system enables synchronous monitoring of ventricular systolic pressure, coronary perfusion pressure, monophasic action potentials, electrocardiograms (ECGs), and perfusion temperature, providing comprehensive electrophysiological and functional assessment.

Principles of Cardiac Electrical Mapping

When the heart is wrapped with flexible microelectrode arrays, multiple electrode sites on the surface can simultaneously record local field potentials. By selecting eight of these sites, eight independent waveforms can be obtained. Because depolarization and repolarization signals occur at different times across sites, combining the multichannel electrophysiological data allows the cardiac electrical activity to be visualized in both space and time. This approach is known as Mapping.

Record Software (Rabbit Heart)

Electrical Mapping System is suitable for cardiac electrical mapping in rabbit hearts. It enables simultaneous acquisition of 128-channel field potential signals from different regions of the heart, while concurrently recording perfusion pressure, systolic pressure, electrocardiograms (ECG), and monophasic action potential (MAP) changes, providing comprehensive access to multiple relevant electrophysiological parameters.

Cardiac Samples From Different Animal Species

Ex vivo zebrafish heart recordings

Ex vivo rat heart recordings

Ex vivo pig heart recordings

Ex vivo shark heart recordings

In Vivo Rat Heart Recordings With Vagus Nerve Stimulation

 

For in vivo rat heart recordings with vagus nerve stimulation, enabling simultaneous monitoring of cardiac ECG and field potential signal changes.

Electrical Mapping of The Porcine Heart In Vivo
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