Amgen and its partners published “Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates” in Communications Biology.Using the high-resolution fluorescence mapping system OMS-PCIE-2002, by culturing hiPSC-CMs in a 24-well plate, loading dyes, and mapping calcium transient signals.

 

Calcium transients and calcium cycling proteins in hiPSC-CMs treated with human insulin (a) Representative Ca 2+ transient measurements during spontaneous beating (SB) and at 1Hz, 1.5Hz, and 2Hz electrical pacing. (b) Representative superimposed Ca 2+ transient traces in hiPSC-CMs without/with human insulin treatment. (c) Mean amplitudes of Ca 2+ transients in hiPSC-CMs exposed to human insulin and CTL during spontaneous beating (SB) and at 1Hz, 1.5Hz, and 2Hz electrical pacing. 4 animals per group.

[1] Liu M, Li N, Qu C, Gao Y, Wu L, Hu LG. Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol. 2021 Feb 12;4(1):188. doi: 10.1038/s42003-021-01676-3. PMID: 33580152; PMCID: PMC7881154.