The most significant feature that distinguishes cardiomyocytes from other cells is that they can beat autonomously, Observing their electrophysiological function changes will help to reveal the new mechanism of heart injury induced by inflammation. CaTD90, which refers to the 90 % duration of calcium transient. The results showed that compared with the group C, the Ca2+ activation time decreased first and then increased at 3 h and 6 h after microwave radiation (p < 0.001), and the Ca2+ activation velocity decreased significantly at 1 h and 3 h (p < 0.001). The Ca2+ transient amplitude decreased and increased at 1 h, 3 h and 6 h after microwave radiation in the group R, and the Ca2+activation amplitude increased at 1 h, 3 h and 6 h after microwave radiation. The dynamic changes of decline (p < 0.01 or p < 0.001); The CaTD90 duration first increased and then decreased (p < 0.001). Meanwhile, the waveform diagram intuitively reflects the above changes.

Fig. 2. Electrophysiological examination of iPSC-CMs after microwave irradiation. A: Waveform of Ca2+ conduction at 1 h after microwave irradiation; B: Ca2+conduction waveform at 3 h after microwave irradiation; C: Ca2+ conduction waveform at 6 h after microwave irradiation; D: Heat map of Ca2+ conduction after microwave irradiation; E: activation time statistics; F: conduction velocity statistics; G: calcium transient amplitude statistics; H: CaTD90 statistics. Group C: control group; Group R: microwave irradiation group. **p < 0.01, ***p < 0.001; n = 5.

Ecotoxicol Environ Saf.

2025 Sep 15;303:119067.

doi:10.1016/j.ecoenv.2025.119067.

Epub 2025 Sep 17. PMID: 40967018.