Schematic Diagram of the Optical Path

Optical Path

Optical Mapping System and Accessories

Dye loading of the heart

Simultaneous Acquisition of AP and Ca Transient

From Tens of Thousands of Channels

Amgen and its partners published "Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates" in Communications Biology.Using the high-resolution fluorescence mapping system OMS-PCIE-2002, by culturing hiPSC-CMs in a 24-well plate, loading dyes, and mapping calcium transient signals.

A, B: The recorded cell area and the placement position of the stimulation

C: Time curves of intracellular calcium conduction and the distribution map of calcium transient duration before and after drug intervention in iPSC-CMs

Cells were inoculated in a four-well plate, incubated with Fluo4 for staining, and subjected to different frequencies of high-frequency stimulation. The electrical signals of the cell layer and the intracellular calcium signals were recorded.