To investigate the impact of WK on RyR2 and SERCA function, we employed a Calcium Contractility Mapping System. This single-cell approach provided high-resolution data on calcium handling and contractility, complementing our tissue-level optical mapping. By applying a specialized protocol to differentiate calcium release and uptake mechanisms, we were able to uncover the specific effects of WK on these critical calcium-handling proteins.

(I) Graphical representation of SR Ca2+ fluxes estimation protocol [a: basic Ca2+ transient parameters, b: RyR Ca2+ leak, c: SR Ca2+ content, d: SERCA activity; NT, normal Tyrode solution.

(J, K) Quantification of RyR2 and SERCA functions of rate-constants.

Theranostics(IF 13.3)

Liang Y, Wang G, Fan S, et. Brain-to-heart cholinergic synapse-calcium signaling mediates ischemic stroke-induced atrial fibrillation. Theranostics. 2024 Oct 7;14(17):6625-6651.

doi: 10.7150/thno.99065.