This system loads calcium (or other ion) fluorescent dyes into test cells, where the dyes enter the cytoplasm, bind to intracellular calcium ions, and alter their fluorescence intensity with calcium concentration fluctuations. A high-speed scientific-grade CMOS camera captures these dynamic signal changes in real time. The system also features single-cell geometric measurement, enabling simultaneous recording of cellular calcium signals and physical contractile changes. Both signals are converted to digital data and sent to a computer for recording, storage, and analysis.

Optical Path

Recording Samples

Recording and Analysis Software

During contraction, the spacing of the alternating dark and light bands formed by the A-band and I-band of the cell shortens or lengthens periodically. By analyzing the periodic arrangement of these bands in the image and calculating their dominant spatial frequency using the Fast Fourier Transform (FFT) algorithm, the average sarcomere length can be accurately determined.

Caffeine assay is a commonly used experimental method in cell biology, mainly applied to study intracellular calcium signaling mechanisms, particularly the calcium release capacity of intracellular calcium stores (such as the sarcoplasmic reticulum) and related signaling pathways (e.g., the ryanodine receptor pathway).

1.Calcium transient + SR Ca²⁺ store

2.Calcium transient + SR Ca²⁺ store + SR Ca²⁺ leak + RyR/SERCA function + PMCA activity

3.Calcium transient + SR Ca²⁺ store + SR Ca²⁺ leak + RyR/SERCA function + PMCA activity + NCX activity