Schematic Diagram of the Optical Path
Optical Path
Optical Mapping System and Accessories
Simultaneous Acquisition of AP and Ca Transient From Tens of Thousands of Channels
An efficient platform for cardiac drug screening and mechanistic studies was established by combining living myocardial slice and mapping techniques. The 300–400 μm ultrathin slices retain the native myocardial structure, support immersion culture for up to one month under sterile conditions, and are compatible with experimental (porcine) and clinical human myocardial tissues.
A ) Calcium transient mapping of porcine ventricular myocardial slices was performed under control conditions, 1 μM cisapride treatment, and co-treatment with 1 μM cisapride plus 10 nM ATXII. The results showed a CaT90 of 289 ms in the control group, prolonged CaT90 (318 ms) with cisapride, and prominent early afterdepolarizations (EADs) in the co-treatment group.
B ) Quantitative analysis of CaT90 distribution in porcine ventricular myocardial slices was conducted for the control, cisapride, and cisapride+ATXII groups. The data demonstrated a concentrated CaT90 distribution around 300 ms in controls, a rightward shift with cisapride, and a further shift accompanied by EAD-associated signals in the co-treatment group.
