Optical Mapping Systems.

Illuminate the electrical activity of the heart.

Introduction.

  • Detect area: 5 to 30 cm
  • Maximum resolution: 512*512 pixels.
  • Dynamic range up to 50,000:1(94 dB)
  • Sampling rate up 3.5 kHz with resolution 32*32 pixels.
  • ROI frame rate: up to 3.5 kHz.
  • 12 bit depth per pixel.
  • TTL synchronization precision: higher than 100μs.
  • Time-lapse imaging.
  • Customized ancillary devices
  • USB 3.0 or PCIE

MappingLab Ltd offers standard OMS-PCIE-1001, OMS-PCIE-1002 and OMS-PCIE-2002 systems as well as customized systems. Dual signal optical mapping systems are suitable for simultaneous recording of cardiac membrane potential and calcium imaging. Our latest optical mapping system is equipped with back-illuminated scientific CMOS cameras with 95% quantum efficiency (QE) and pixel size from 6.5 μm x 6.5 μm to 11 μm x 11 μm. It provides high-speed, low-noise acquisition with a dynamic range of up to 50,000:1 (94 dB). The optical axis can be either vertical or horizontal. There are various I/O connectors to help researchers set up the communication system.  An 8-channel TTL controller is designed to synchronize devices such as camera, LED light controller, stimulator, ECG, MAP, pressure and temperature probes with the optical mapping system. OMapRecord/Scope is an advanced data acquisition and analysis software with a user-friendly intuitive interface and highly efficient data management.

Software.

OMapRecord can simultaneously record a variety of fluorescence signals, including membrane potential and calcium transient. It can also record ECG and pressure at the same. OMapScope is a powerful analysis software with a user-friendly interface, which can compute many important and meaning parameters, such as conduction velocity, conduction dispersion, AP triangulation, APD, and phase mapping.

Record

OMapRecord 5

Analysis

OMapScope 5

Hardware.

OMS-PCIE-1001 and OMS-PCIE-1002 have a CMOS camera built in. The difference between them is that the former can only acquire a single fluorescence signal, i.e. calcium or membrane potential, while the latter can acquire two fluorescence signals simultaneously. However, OMS-PCIE-2002 has two CMOS cameras in the system to acquire calcium and membrane potential simultaneously. In principle, OMS-PCIE-2002 is the better choice for dual signal recording due to higher resolution and less signal attenuation.

OMS-PCIE-1001

OMS-PCIE-1002

OMS-PCIE-2002

Ancillary Devices for Optical Mapping System.

Optical Mapping System Controller

LEDC-2001 LED Controller

Heart/Tissue Perfusion Chamber

VCS-3001 Stimulator