The calcium and contractility mapping system is designed to synchronously monitor myocardial contractions and cellular calcium transient signals. In addition, this system is capable of detecting action potentials and calcium transient signals in small cardiac models (such as isolated zebrafish hearts) and organoids, ranging in size from 0.1 mm to 3 mm.
Parameters
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Calcium signals: calcium transient duration (CTD10-90), CTD10-90 dispersion, peak time, recovery time, amplitude, intracellular calcium conduction, and video visualization of intracellular calcium activity.
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Contraction: contraction and relaxation times, excitation-contraction coupling time.
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Action potential (organoids and zebrafish): action potential conduction time, direction, velocity, dispersion, depolarization rate, peak depolarization time, action potential duration (APD10-90), APD10-90 dispersion, and video visualization.
Application
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Assessment of drug-induced toxicity by evaluating drug effects on various cardiac parameters to assess
cardiac safety. -
Monitoring the pharmacodynamics of antiarrhythmic drugs, both traditional and modern Chinese medicines, for screening purposes.
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Characterization of organoid properties.
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To study calcium handling and contractile differences.
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Investigate calcium waves in cardiomyocytes and arrhythmia mechanisms.
Applicable Samples
Acutely isolated cardiomyocytes and their monolayers, iPSC-CMs, spheroids, organoids, smooth muscle cell monolayers, skeletal muscle cell monolayers, cardiac slices, and small-sized hearts (e.g., isolated zebrafish hearts).
Hardware
The main control unit, constant current or voltage stimulator, temperature controlling system, eight-channel perfusion system, microscopic imaging device, optical path system, LED excitation light source.
