The most significant feature that distinguishes cardiomyocytes from other cells is that they can beat autonomously, Observing their electrophysiological function changes will help to reveal the new mechanism of heart injury induced by inflammation. CaTD90, which refers to the 90 % duration of calcium transient. The results showed that compared with the group C, the Ca2+ activation time decreased first and then increased at 3 h and 6 h after microwave radiation (p < 0.001), and the Ca2+ activation velocity decreased significantly at 1 h and 3 h (p < 0.001). The Ca2+ transient amplitude decreased and increased at 1 h, 3 h and 6 h after microwave radiation in the group R, and the Ca2+activation amplitude increased at 1 h, 3 h and 6 h after microwave radiation. The dynamic changes of decline (p < 0.01 or p < 0.001); The CaTD90 duration first increased and then decreased (p < 0.001). Meanwhile, the waveform diagram intuitively reflects the above changes.
A: Representative Ca2+ transient measurements at spontaneous beating (SB) and electrically paced at 1Hz, 1.5Hz and 2Hz, respectively. (b) Representative superimposed Ca2+ transient traces in hiPSC-CMs treated without/with human amylin. (c) Mean amplitude of Ca2+ transients in hiPSC-CMs exposed to human amylin and CTL at spontaneous beating (SB) and electrically paced at 1Hz, 1.5Hz and 2Hz, respectively (n = 4/group). (d) Illustrative isochronal maps of calcium activation time, calcium amplitude and at different frequency. In the activation time map, warmer color means shorter activation time. In the amplitude map, blue color represents higher amplitude. In the CaD90 (the duration of calcium transient at 90% decay) map, red color means shorter duration.
Communications biology (IF 5.1)
Liu M, Li N, Qu C, Gao Y, Wu L, Hu LG. Amylin deposition activates HIF1α and 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) signaling in failing hearts of non-human primates. Commun Biol. 2021 Feb 12;4(1):188. doi: 10.1038/s42003-021-01676-3. PMID: 33580152; PMCID: PMC7881154.
