Isolated (Ex Vivo) Heart
The ex vivo whole-heart model retains the complete myocardial tissue structure, electrical conduction network, and excitation–contraction coupling system. It can faithfully simulate the in vivo physiological functions of the heart, compensate for the lack of tissue microenvironment in single-cell models, and serve as a key model for cardiac physiological and pathological mechanism research and preclinical translation. It can be used in research on myocardial infarction, arrhythmia, heart failure, and other diseases to clarify the mechanisms underlying electrical signal abnormalities, calcium homeostasis imbalance, and functional impairment. Meanwhile, it is applied to the cardiac safety evaluation of drugs, screening of anti-arrhythmic drugs, and efficacy evaluation of regenerative therapies such as stem cell transplantation.
In the Mapping Lab mapping system, this model can simultaneously collect electrical activity, calcium transients, and contractile function signals via optical mapping and electrical mapping, accurately characterize key indicators, and distinguish normal from pathological regions. Combined with optoelectrical hybrid detection, it can systematically evaluate changes in excitation–contraction coupling, provide high-dimensional and quantitative data support for relevant research and translation, and serve as an important platform connecting basic research and clinical application.
Application of Optical Mapping System in the Ex Vivo Whole Heart
Schematic Diagram of the Optical Path Optical Path Optical Mapping System and Supporting Equipment Dye Loading in the Ex Vivo Whole Heart Simultaneous Acquisition of Action Potential (AP) and Calcium Transi ...
Application of Electrical Mapping System in the Ex Vivo Whole Heart
Electrical Mapping System Using multi-site recording electrodes placed in direct contact with excitable tissues or cells, electrical signals are acquired simultaneously from multiple locations. Dedicated software the ...
【Science Bulletin】Initial cardioplegic flush with crystalloid cardioplegia improves donor heart preservation and function via NR4A3 upregulation and metabolic reprogramming
In electrical mapping experiments on Langendorff-perfused rat hearts, the control group and the group treated with crystalloid cardioplegia (CC) combined with University of Wisconsin (UW) preservation solut ...
【Regenerative Biomaterials】Enhanced prevention on postoperative atrial fibrillation by using anti-inflammatory biodegradable drug patch
In a sterile pericarditis (SP) rat model, in vivo electrical mapping revealed significant atrial conduction heterogeneity in both the SP and blank patch groups. (a) A schematic illustrating the experimental ...
【Nature Communications】Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions
MI frequently precipitates arrhythmias by impairing CM function and inducing fibrosis, which impedes cardiac electrical signal propagation. Utilizing cardiac optical mapping with calcium-sensitive dye, they ...
【Nature Biomedical Engineering】Injectable and conductive cardiac patches repair infarcted myocardium in rats and minipigs
The electrical mapping was performed on the isolated Langendorff-perfused rat hearts after the patches were implanted onto the infarcted hearts for 4 weeks. These results provide evidence that the conductiv ...
【JACC. Clinical electrophysiology 】Low-Dose Cardiac Radiation Improves Electrical Function and Reduces Ventricular Arrhythmogenesis in Mice With Nonischemic Cardiomyopathy
Results from left ventricular epicardial electrical mapping showed that, compared to sham-operated heart failure mice, the treatment group exhibited shorter ventricular activation time and lower activation ...
【Exploration】Ion cocktail therapy for myocardial infarction by synergistic regulation of both structural and electrical remodeling
Using optical mapping to analyze action potentials in different regions of the heart (normal zone (N), peri-infarct zone (P), and infarct zone (I) of the myocardial infarction heart), it was found that comp ...
【Clinical and translational medicine】Enhanced human adipose-derived stem cells with VEGFA and bFGF mRNA promote stable vascular regeneration and improve cardiac function following myocardial infarction
The electrical mapping system was used to detect changes in left ventricular electrical conduction at 15 minutes and 4 weeks after myocardial infarction (MI). Conduction time (CT), conduction velocity (CV), ...
【Circulation】Modeling Long-QT Syndrome Using Gene-Edited Miniature Pigs
Using electrical mapping technology, ventricular tachycardia was induced and recorded in vivo. The results showed that under the same stimulation conditions, the hearts of wild-type pigs were successfully p ...
【Cardiovascular research】Senescent CD8+ T cells: a novel risk factor in atrial fibrillation
To further clarify the potential role of IFN-γ in susceptibility to atrial fibrillation, the researchers performed optical mapping on rat hearts. Although IFN-γ perfusion did not significantly alter the atr ...
【Cardiovascular research】Coupling of USP10 de-ubiquitination and chaperone-mediated autophagy causes cardiac sodium channel degradation and cardiac arrhythmias
Optical mapping confirmed prolonged activation time and reduced conduction velocity in both sinus rhythm and pacing modes. Programmed electrical stimulation experiments demonstrated a high incidence of indu ...
【Theranostics】Brain‑to‑heart cholinergic synapse‑calcium signaling mediates ischemic stroke‑induced atrial fibrillation
In this study, an atrial fibrillation rat model induced by ischemic stroke was established. Optical mapping technology was employed to detect action potentials and calcium transient signals in different mod ...
【Biomedicine & pharmacotherapy 】Sanwei sandalwood decoction ameliorates acute ischemia-reperfusion injury in rats by modulating myocyte electrophysiological characteristics
The Sanwei Tanxiang decoction powder slowed the heart rate and conduction velocity in normal rats; significantly prolonged ventricular repolarization time and effective refractory period, and mildly extende ...
【Journal of Ethnopharmacology】Traditional Chinese Medicine YangxinDingji alleviates arrhythmias through inhibition of sodium and L-type calcium channels
Using optical mapping technology on isolated hearts, this study investigated the effects of Yangxin Dingji Capsule on electrical and calcium disturbances in heart models stimulated by isoproterenol (ISO). I ...
【Advanced science】P21-Activated Kinase 2 as a Novel Target for Ventricular Tachyarrhythmias Associated with Cardiac Adrenergic Stress and Hypertrophy
Using optical mapping, electrophysiological changes were compared between Langendorff-perfused Pak2cko and Pak2f/f hearts. Under baseline conditions, there was no difference in the incidence of ventricular ...
【Advanced Materials】Pericardial Delivery of SDF-1 a Puerarin Hydrogel Promotes Heart Repair and Electrical Coupling
The contraction and relaxation of cardiomyocytes rely on periodic action potentials, a process closely associated with calcium. Impaired myocardial function is partly attributed to alterations in excitation ...
