Cardiomyocytes serve as the primary cellular model for studying heart diseases. Isolating individual cardiomyocytes from the heart eliminates vascular and neurohormonal influences, allowing the investigation of pharmacological, genetic, or surgical interventions on cellular physiology at the microscopic level. As the basic functional units, cardiomyocytes are responsible for myocardial tissue contraction.Cardiomyocytes possess a complete excitation-contraction coupling (ECC) system and the sarcomeric structures that mediate cell contraction. Excitation-contraction coupling refers to the process by which an action potential triggers Ca²⁺ release from the sarcoplasmic reticulum, which subsequently induces cardiomyocyte contraction. Through this mechanism, the electrical energy of the action potential is converted into the mechanical energy of cardiac contraction, enabling the heart to perform work and generate macroscopic function. Intracellular Ca²⁺ regulation plays a central role in this process, making simultaneous measurements of calcium dynamics and contraction particularly critical for cardiac research.Measuring and analyzing calcium signals and contraction in isolated cardiomyocytes helps to better elucidate cardiac physiological and pathophysiological mechanisms. The Cell Contraction and Calcium Imaging Mapping System represents a powerful tool for detecting cardiomyocyte contraction and calcium signaling.