Atrial Fibrillation
In Atrial fibrillation (AF) is the most prevalent sustained arrhythmia globally. Its pathogenesis involves a complex interplay of electrical, structural, calcium-handling, and autonomic remodeling, representing a functional disorder that spans a continuum from molecules and ion channels to cells, tissues, and the whole organ. Current therapies remain limited: antiarrhythmic drugs are often ineffective or pro-arrhythmic, while catheter ablation still fails in 30–50% of persistent AF cases, largely due to the inability to precisely locate AF drivers. Bridging the gap between basic research and clinical translation requires a systematic, multi-scale understanding of AF pathophysiology.
AF is multifactorial, and current therapies often underperform, partly due to difficulty in pinpointing dynamic drivers. MappingLab integrates optical, electrical and calcium for synchronised multi scale electrical/Ca²⁺acquisition, supporting human models, optogenetics, CiPA screening, and ablation testing, with modular expandability for cross disciplinary research—accelerating mechanistic discovery and therapeutic translation for AF.
Application of Optical Mapping System in Atrial Fibrillation
Schematic Diagram of the Optical Path Optical Path Optical Mapping System and Accessories Dye loading of the heart Simultaneous Acquisition of AP and Ca Transient From Tens of Thousands of Channels Optica ...
Application of Electrical Mapping System in Atrial Fibrillation
Electrical Mapping System This technique utilizes multi-site recording electrodes to directly contact excitable tissues or cells, enabling the synchronous recording of signals from multiple locations. Through softwar ...
【Translational Research】Cardiac-specific overexpression of CREM-Ib△C-X via CRISPR/Cas9 in mice presents a new model of atrial cardiomyopathy with spontaneous atrial fibrillation
Atrial electrophysiological remodeling in the CS-CREM mice The representative ECG and the RR interval distributions were shown in Figure 3A and Supplementary Figure 2, respectively. Monthly ECG tests reveal ...
【Theranostics】Brain‑to‑heart cholinergic synapse‑calcium signaling mediates ischemic stroke‑induced atrial fibrillation
As shown in the experimental scheme in Figure 1A, after 60 min ischemia, rats were treated with different doses of WK or a positive control drug Met for 14 days. ECG from Sham, Model, WK-L, WK-M, WK- ...
【The Royal Society Publishing】Late sodium current in synergism with Ca2+/calmodulin-dependent protein kinase Il contributes to β-adrenergic activation-induced atrial fibrillation
Infusion with 15 nM ISO increased the IHI from 1.2 ± 0.03 to 1.7 ± 0.03 at constant pacing rate (4.5 Hz, n = 6, p < 0.05, figure 3a). Compared with an orderly spread of excitation beginning with localize ...
【The Journal of Clinical Investigation】Modulation of NOX causes obesity-mediated atrial fibrillation
NOX2 inhibition prevents obesity-mediated atrial fibrosis and increases atrial CV. To study changes in conduction, epicardial multielectrode array mapping of both atria and the left ventricle was performed ...
【Redox Biology】Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress
To evaluate AF susceptibility, we carried out a transvenous burst pacing to induce atrial fibrillation. After programmed stimulation, compared to the WT + AAV-CON (1/10) and WT + AAV-Chrna7 (1/10) groups, a ...
【Journal of the American Heart Association】Mkk4 Is a Negative Regulator of the Transforming Growth Factor Beta 1 Signaling Associated With Atrial Remodeling and Arrhythmogenesis With Age
Epicardial multielectrode array (MEA) mapping of both left and right atrial conduction properties were performed during either sinus rhythm or PES, in isolated Langendorff‐perfused hearts. Figure 3A exempli ...
【JCI Insight】TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats
The propensity to atrial arrhythmia was also assessed in isolated hearts using optical mapping and S1S2 stimulation. Representative optical AP traces and corresponding ECG are shown in Figure 3, A–C, ...
【Europace】N-palmitoyl glycine mediates depression-associated atrial fibrillation via Man1a2-dependent autophagy and NRAP dysregulation
To detect the influence of this pathway on atrial electrical activity and whether the above pathway works through sympathetic and parasympathetic nerves, a Electrical cardiac electrical mapping technique wa ...
【BBA-Molecular Basis of Disease】Icariin attenuates excessive alcohol consumption-induced susceptibility to atrial fibrillation through SIRT3 signaling
After 12 weeks of alcohol intake, the mice were subjected to atrial burst pacing. Alcohol treatment increased the inducibility and duration of AF (Fig. 1B–D). Further atrial electrical mapping results revea ...
【Biomedicine & Pharmacotherapy】Colchicine prevents atrial fibrillation promotion by inhibiting IL-1ß-induced IL-6 release and atrial fibrosis in the rat sterile pericarditis model
To examine the inhibition of IL-6 involved in the effect of colchicine in preventing the occurrence of POAF, we administered IL-6 to rats to determine whether the therapeutic eff ;ect of colchicine can be a ...
【Free Radical Biology and Medicine】Activation of PKG-CREB-KLF15 by melatonin attenuates Angiotensin Il-induced vulnerability to atrial fibrillation via enhancing branched-chain amino acids catabolism
As shown in Fig. 2A and B, Ang II-infusion increased the inducibility and duration of AF, while this was attenuated by BT2 treatment. Meanwhile, the electrophysiological mapping experiment showed that Ang I ...
【Life Sciences】Activation of NADPH oxidase mediates mitochondrial oxidative stress and atrial remodeling in diabetic rabbits
The results of the epicardial mapping of right and left atria in three groups of rabbits are shown in Fig. 3. Mapping images of the right atrium in three groups are shown in panels a–c, whilst graphs showin ...
【Circulation: Arrhythmia and Electrophysiology】Signal Transducer and Activator of Transcription 3/MicroRNA-21 Feedback Loop Contributes to Atrial Fibrillation by Promoting Atrial Fibrosis in a Rat Sterile Pericarditis Model
No significant differences were observed in the parameters examined. Typical ECG recordings before and after transesophageal burst pacing are presented in Figure 1A and 1B. To evaluate the atrial conductio ...
【Cellular and Molecular Life Science】Prostaglandin I2 signaling prevents angiotensin Il-induced atrial remodeling and vulnerability to atrial fibrillation in mice
The duration of AF was shortened substantially in mice administered iloprost compared with vehicle-treated mice following Ang II infusion (Ang II+vehicle: 2.962±0.081 s versus Ang II+iloprost: 1.747±0.092 s ...
【Cardiovascular Research】Senescent CD8+ T cells: a novel risk factor in atrial fibrillation
To further elucidate the potential role of IFN-γ in AF susceptibility, we performed optical mapping of the heart using rats. Although IFN-γ perfusion did not significantly alter AERP or APD, it precipitated ...
【Antioxidants & Redox Signaling】Anthracycline-Induced Atrial Structural and Electrical Remodeling Characterizes Early Cardiotoxicity and Contributes to Atrial Conductive Instability and Dysfunction
By using electrical mapping (Figure 2D), we found that doxorubicin slowed down the conduction velocity by 2.2 folds (Figure 2E), increased the dispersion index by 3.6 folds (Figure 2F) and prolonged the act ...
