Cardiac optical mapping has become an important technique using fluorescent-imaging technique in the study of the electrical properties of multicellular cardiac preparations, especially in terms of investigating the mechanisms of cardiac arrhythmias. It is applicable to study isolated hearts, intact cardiac tissue preparations, muscle slice and monolayers of pluripotent stem-cell derived or neonatal cardiac myocytes. Optical mapping is assisted with use of voltage-sensitive dyes that track changes in the potential difference across the cell membrane of the cardiac myocytes whilst with fluorescent-microscopy to image the spread of electrical waves (using calcium indicators) throughout the heart. Due to its potential to generate high spatiotemporal resolution information on electrophysiological properties, optical mapping is a popular experimental technique which has been used to interrogate the fundamental mechanisms governing the electrical stability of the heart in health and disease.
Simultaneous recording of action potential and calcium transient from a Langendorff rabbit heart
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